Easy and Rapid Live Bacteria Counter by Flow Cytometry
نویسندگان
چکیده
منابع مشابه
Multiparameter flow cytometry of bacteria.
The small size of bacteria makes some microbial constituents undetectable or measurable with only limited precision by flow cytometry. Bacteria may also behave differently from eukaryotes in terms of their interaction with dyes, drugs, and other reagents. It is therefore difficult to design multiparameter staining protocols that work, unmodified, across a wide range of bacterial species. This c...
متن کاملDetection, Identification and Susceptibility Testing of Bacteria by Flow Cytometry
With the multiparametric measurement of individual cells at a rate of several thousand per second, flow cytometry offers a rapid method for the detailed analysis of microorganisms. This technique provides quantitative data for cell size or granularity through light scattering signals and via fluorescence signals quantitative information on the expression of surface antigens, or intracellular co...
متن کاملDetermination of DNA content of aquatic bacteria by flow cytometry.
The distribution of DNA among bacterioplankton and bacterial isolates was determined by flow cytometry of DAPI (4',6'-diamidino-2-phenylindole)-stained organisms. Conditions were optimized to minimize error from nonspecific staining, AT bias, DNA packing, changes in ionic strength, and differences in cell permeability. The sensitivity was sufficient to characterize the small 1- to 2-Mb-genome o...
متن کاملA flow cytometry method for rapid detection and enumeration of total bacteria in milk.
Application of flow cytometry (FCM) to microbial analysis of milk is hampered by the presence of milk proteins and lipid particles. Here we report on the development of a rapid (/= 0.98) between the FCM assay and the more conventi...
متن کاملRapid susceptibility testing of Mycobacterium tuberculosis (H37Ra) by flow cytometry.
The resurgence of tuberculosis has caused considerable effort to be focused on the development of rapid methods for determining the susceptibility of Mycobacterium tuberculosis to antimycobacterial agents. We demonstrated that susceptibility testing of M. tuberculosis can be accomplished rapidly by using flow cytometry. Results of tests were available within 24 h after M. tuberculosis organisms...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Japan Journal of Food Engineering
سال: 2013
ISSN: 1345-7942,1884-5924
DOI: 10.11301/jsfe.14.131